Streptavidin Horseradish Peroxidase Conjugates: Tech Data


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HORSERADISH PEROXIDASE CONJUGATES

PRODUCT	CODE	QUANTITY
Streptavidin-HRP (ELISA) - a high signal specialty conjugate optimized for ELISAs.	CJ30H	> 3 ml
Streptavidin-HRP (IHC) - a specialty conjugate with lower average size designed specifically for immunohistochemistry.	CJ31H	> 3 ml

Each conjugate has been purified to remove unreacted components in order to improve sensitivity and reduce non-specific binding. The conjugates are supplied in a mercury-free, stabilized solution and are stable as supplied for at least 12 months when properly stored. A loss of activity may be observed over the course of several weeks after dilution. Shipped with an ice pack for next day delivery.

Storage:

4°C. DO NOT FREEZE

Inhibitors: Inhibitors of horseradish peroxidase activity include azide, fluoride, cyanide and sulfide ions. In addition, proteins or other compounds which react directly with hydrogen peroxide will interfere with color development; these include catalase, ascorbate, bilirubin and hemoglobin.
Detection for ELISA: Many substrates and protocols are used (for example, 34 mg o-phenylenediamine per 100 ml 0.1 M citrate buffer pH 5.0, containing 0.012% H₂O₂). The development reaction may be stopped with 10 µl of 6 N HCl per 100 µl of substrate. This reaction yields a soluble product with an absorption maximum at 492 nm (Tijssen, 1985). Alternatively, a substrate which yields an insoluble product may be employed. Again, many alternatives exist. For example, a substrate solution may be prepared by mixing 40 ml of 50 mM sodium phosphate pH 7.0, containing 0.15 M sodium chloride with 5 ml of methanol containing 10 mg of 3-3' diaminobenzidine and 30 mg of 4-chloronaphthol. Just before use, 10 µl of 30% hydrogen peroxide is added.

Origin: USA

REFERENCES
Kincaid, R. L. and M. S. Nightingale. A rapid non-radioactive procedure for plaque hybridization using biotinylated probes prepared by random primed labeling. BioTechniques 6:42-49 (1988)
O'Shannessey, D. J., Voorstad, P. J. and R. H. Quarles. Quantitation of glycoproteins on electroblots using the biotin-streptavidin complex. Analyt. Biochem 163:204-209 (1987).
Tijssen, P. Practice and Theory of Enzyme Immunoassays. (H. Burden and P. H. van Knippenberg, eds.). Elsevier Science Publishers, Amsterdam The Netherlands (1985).

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