• Minimal sialidase with extended digestion times (up to 3 hours) for cost-effective analysis
• Single-use kit (30 data points) for small numbers of samples

For quantitation of total sialic acid by fluorescence detection, either free or released from intact glycoproteins using minimal amounts of sialidase with extended digestion times for enzymatic release of sialic acid:

•  Broad range of detection, 40 pmol - 1,000 pmol

•  96-well microplate format with multichannel pipettor

• Sample digestion, conversion, detection and quantitation performed in a single well for fast and simple processing

• Enzymatic cleavage with minimal, if any, degradation of sialic acid

• Sufficient for ~30 total data points (sialic acid standard and 4 samples in duplicate)

[booklet] abridged

[TNGS300.1 An Enzyme-based Sialic Acid Quantitation Assay for Rapid Screening of Therapeutic Glycoproteins During Process Development:  A Potential Process Analytical Technology]

[TNGS300.2 Qualification of a Process Analytical Technology for Quantifying Sialic Acid on Therapeutic Proteins using Two Instrument Platforms]