Glyko Tools for Glycobiology
 
 
 
N-Glycanase® (rPNGase F)
Code Description Size/Qty Price
GKE-5006A
 

GKE-5006B
 
 
 
 
 

GKE-5006D
 
 

 

N-Glycanase® (recombinant gene from Elizabethkingia meningoseptica, expressed in E. coli)

NOTE:  The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum

Purchase includes a limited license to use.

[Peptide-N-Glycosidase F, PNGase F], cleaves all asparagine-linked complex, hybrid or high mannose oligosaccharides unless alpha(1-3) core fucosylated; asparagine must be peptide-bonded at both termini.  Phosphate, sulfate and sialic acid groups attached to the oligosaccharide do not affect cleavage. Endo F free.

“A & B” pack sizes ship with WS0010 5x N-Glycanase Reaction Buffer, 
WS0012 Denaturation Solution & WS0013 Detergent Solution

Not formulated for FACE® Analysis, use GK50050 instead

Tech DataFAQ
TechNote  TNGK200.1Comparing PNGase F Activity

(Buffer & solutions not included with GKE-5006D & GKE-5006E)

100 mU
(40 µl)

200 mU
(80 µl)
 
 
 
 
 

1 unit
 
 

 

$244
 

$446
 
 
 
 
 
 

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Rev. 12/05/07