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I am using a Streptavidin agarose system to affinity purify biotin labeled, outer membrane proteins of a gram negative bacterium. Could you provide info regarding preparation of buffers and the reaction and elution conditions of this system?
 

The high affinity of streptavidin for biotin precludes using streptavidin-agarose for binding and eluting biotinylated proteins, because there is no way to elute the proteins without denaturing them  and the streptavidin.

Streptavidin-agarose has been used for affinity purification, by binding a biotinylated affinity ligand.  Examples include binding biotinylated antibodies to streptavidin-agarose for immunoaffinity purification of
antigens.

Refer to our Streptavidin-agarose Technical Data Sheet (Product Code CJ30R).  Several examples of such procedures are cited.

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