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I need more of the desalting columns (A & B) that are supplied with your kits.  Where can I get them?  Can they be re-used/regenerated?

Desalting columns may be purchased from Amersham Pharmacia Biotech (catalog #17-0851-01).  Initially we used columns from Pierce Chemical (catalog #43232), so you may see some difference in the appearance from each supplier.  Column material is cross-linked dextran.  Please note that the columns must be equilibrated before use.

Columns may be re-used several times.  To prepare a column for initial use or re-use, simply wash it with 5 - 10 column volumes of the appropriate buffer (recipes for buffers used in the Conjugation Protocol may be found in the Appendix* in the package insert).  We have used these columns 2 - 4 times over the course of a day, but we haven’t kept used columns for longer periods.  You might be able to do so, but microbial growth is a concern.

*PJ25K Allophycocyanin kit booklet used in this example. Same column preparation used for PJ31K.

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