Home| News | About Us | Tech Support | Orders |Products | Terms | Directions | Contact Us

What Glyko products do I need for glycan profiling of an antibody by HPLC using 2-AB fluorescent label?
 

We Recommend:

GKE-5010 N-Glycanase® Plus for deglycosylation of the Mab.  See FAQ on our website at:

http://www.prozyme.com/faqs/gke-5010faq.pdf

Product listing and tech sheet:

http://www.prozyme.com/pdf/gke-5010.pdf

GKI-4756 GlycoClean™ R Cartridge, optional for cleanup of the glycan samples (protein removal) if you have problems with the ethanol precipitation, due to the limited amounts of the protein or its solubility in ethanol:

http://www.prozyme.com/glyko/columns.html#glycoclean

The product insert is posted on our website:

http://www.prozyme.com/pdf/gki-4756.pdf

GKK-404 Signal™ 2-AB Labeling Kit for labeling the glycans:

http://www.prozyme.com/glyko/signal.html#gkk-404

The product insert is posted on our website:

http://www.prozyme.com/pdf/gkk-404.pdf

GKI-4726 GlycoClean S Cartridges are used after 2-AB labeling to remove unreacted dye and salts:

http://www.prozyme.com/glyko/columns.html#glycoclean


 The product insert is posted on our website:

 
http://www.prozyme.com/pdf/gki-4726.pdf
5. GKI-4728 GlycoSep™ N Column with the GKI-4728G N Guard Column are used to separate the 2-AB-labeled glycans from one another (mostly by size).  The product insert is posted to our website:
 
http://www.prozyme.com/pdf/gki-4728.pdf
6. GKSB-503 2-AB-(Glucose Homopolymer) Standard is used to confirm proper functioning of the HPLC column, and to calculate GU numbers to corroborate and identify peaks.
 
http://www.prozyme.com/pdf/c-a/gksb-503-2-ab.pdf
7. 2-AB-labeled Standards are available for glycan identification:
 
http://www.prozyme.com/glyko/carbostandards.html#2-ab
8. Various glycan standards appropriate for monoclonals are:  NGA2, NGA2F, NA2, NA2F, A2 and A2F.  If the MAb is from mouse hybridoma, add NA2FB.

 

 

NGA2 (aka G0) - GKSB-301  2-AB-Asialo-, agalacto-, biantennary

NGA2F - GKSB-302  2-AB-Asialo-, agalacto-, biantennary, core-substituted with fucose

NA2 (aka G2) - GKSB-304  2-AB-Asialo-, galactosylated biantennary

NA2F - GKSB-305  2-AB-Asialo-, galactosylated biantennary, core-substituted with fucose

A2 - GKSB-312  2-AB-Di-sialylated-, galactosylated biantennary

A2F - GKSB-313  2-AB-Di-sialylated-, galactosylated biantennary, core-substituted with fucose

NA2FB - GKSB-306  2-AB-Asialo-, galactosylated biantennary, core-substituted with fucose and with bisecting GlcNAc
 

9. These standards are also available unlabeled for validation of the 2-AB labeling step.  These are also useful as standards for analysis of the cleaved glycans with MALDI-TOF mass spectrometry:
 
http://www.prozyme.com/glyko/carbostandards.html#n-linked-oligo


NGA2 (aka G0) - GKC-004300  Asialo-, agalacto-, biantennary

NGA2F - GKC-004301  Asialo-, agalacto-, biantennary, core-substituted with fucose

NA2 (aka G2) - GKC-024300  Asialo-, galactosylated biantennary

NA2F - GKC-024301  Asialo-, galactosylated biantennary, core-substituted with fucose

A2 - GKC-224300  Di-sialylated-, galactosylated biantennary

A2F - GKC-224301  Di-sialylated-, galactosylated biantennary, core-substituted with fucose

NA2FB - GKC-024311  Asialo-, galactosylated biantennary, core-substituted with fucose and with bisecting GlcNAc
 

10. GKI-4025 GlycoClean H Cartridges for glycan cleanup when MALDI-TOF is employed for analysis.  H cartridges remove buffer salts in the samples.
 
http://www.prozyme.com/pdf/gki-4025.pdf

N-Glycanase® is a registered trademark of Genzyme Corporation, Boston, MA, USA.  Recombinant PNGase F is covered by US Patent No 5,238,821, which is exclusively licensed for manufacture by ProZyme, Inc.

Home | News | About Us | Tech Support | Orders | Products | Terms | Directions | Contact Us
ProZyme® is a trademark of 1933 Davis Street, Suite 207 San Leandro, CA 94577-1258, USA Phone (510) 638-6900     Toll Free (800) 457-9444 (US & Canada only) fax (510) 638-6919  email info@prozyme.com	Copyright © 2005 Rev. 6/28/04