GlykoPrep™ Rapid Glycoprotein Sample Preparation Platform

N-Glycan Sample Preparation

The distribution of N-linked glycans plays a critical role in the efficacy, immunogenicity, and pharmacokinetics of therapeutic proteins. Multi-step, multi-day , sample preparation methods have limited N-linked glycan analysis to only samples that represent critical decision points. Glycan analysis for bioreactor control, strain selection or comparability studies has never been possible.

The GlykoPrep System

The GlykoPrep™ platform dramatically streamlines glycoprotein sample preparation. From purification to glycan release and labeling, the entire sample prep can be completed in 3 hours, not 3–4 days.

• Innovative platform can process up to 192 samples in < 3 hours
• Modular format enables seamless integration into a variety of workflows
• Rapid labeling and cleanup for quantitative data

The GlykoPrep Sample Preparation System requires minimal equipment: a centrifuge with a deep-well plate rotor (minimum 44 mm height), a Starter Labware Kit, and Heater and Incubation Blocks.

New glycoanalysis kits coming soon — call us to talk about our new product pipeline!

Flexibility

GlykoPrep is modular and can be integrated into any workflow, regardless of sample throughput or sample type. The GlykoPrep platform is a streamlined solution for anyone that requires rapid, quantitative glycan profiling on your current analysis platform.

Downloadable Posters

• Assessing the Variability of an Innovator Molecule N-Glycan Profile
• High-throughput Screening for a Biosimilar N-Glycan Profile
• N-Glycan Profiling Using Orthogonal Methods: UPLC® and CE
• An Integrated Strategy for N-Glycan Sample Preparation and Analysis Suitable for All Stages of Therapeutic Protein Discovery, Characterization, Manufacture and Quality Release
• Feasibility of GlykoPrep™ Sample Preparation for Glycoanalysis on the AssayMAP® Bravo® Liquid Handling Workstation
• Rapid Sample Prep for N-Glycan Analysis Using High Throughput Micro Chromatography
• Rapid, High Throughput, High Sensitivity Determinations of MAb Concentration for Cell Line Screening and Process Optimization
• A New Platform for High Throughput N-Glycan Sample Preparation
• Rapid Sample Prep for N-Glycan Analysis Using High Throughput Micro Chromatography.
• On the Path to Biobetter Therapeutic Glycoproteins: Simple and Rapid Domain-Specific Screening to Target and Control Optimal Glycan Profiles
• Improving Standard N-Glycan Sample Preparation with Manual Automation Using Microchromatography to Improve Efficiency, Accuracy, and Reproducibility
• High-Throughput Platform for Preparation of APTS-Labeled N-Glycans: Improving the Accuracy, Reproducibility and Time-to-Results of N-Glycan Profiling
• Rapid Sample Preparation of Biologics to Support High-throughput and High-resolution Glycan Analysis by Capillary Electrophoresis

The GlykoPrep Workflow

• Contact us for a demonstration of the GlykoPrep Rapid Glycoprotein Sample Preparation Platform

*POROS™ and MabCapture™ are registered trademarks of Life Technologies Corporation.

†Acceptable microplate centrifuge/rotor combinations must be able to 1) accept a stack of microtiter plates 44 mm high, and 2) centrifuge accurately at <50xg RCF. The centrifuge/rotor/bucket combinations listed above have these specifications.